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KMID : 0644319980040010177
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Journal of Korean Oriental Oncology
1998 Volume.4 No. 1 p.177 ~ p.197
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Study on the Effect of Yipahnsan(立安散) on Angiogenic Inhibition Mechanism
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李紀龍/Lee, Gi-Ryong
崔昇勳/安圭錫/Choi, Seung-Hoon/Ahn, Kyoo-Seok
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Abstract
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This experimental study waas carried out to evaluate the effect of Yipahnasn on angiogenic inhibition mechanism. This study investigates the effects of Yipahnsan on angiogenic inhibition mechanism evaluate cell adhesive inhibition effect, DNA fragmantation analysis, nuclear condensation assay, FACScan analysis, angiogenic lumen formation assay, immunocytochemistry analysis, RT-PCR cor mRNA expression, western blot analysis, confocal analysis for Ca^2+influx.
The results were wummarized as follows:
1. The cell adhesive inhibition ability was strongly increased from 5ug/ml on ECV304 cell line and ECVPAR cell line.
2. YY water extract caused G_0/G_1 arrest peak to existed on the ECV304 cell line.
3. YY water extract caused inhibition of proliferation and inducement of apoptosis on the collagen coated plate in ECV304 cell line.
4. YY water extract inhibited the lumen formation on the matrigel coated plate in ECV304 cell line.
5. YY water extract inhibited the expressions of LFA-1 and ELAM-1 on ECV304 cell line and ECVPAR cell line.
6. YY water extract inhibited the expressions of MMP-9 and uPA on ECV304 cell line and ECVPAR cell line.
7. YY water extract inhibited the expressions of integrin α_vβ_3 on ECV304 cell line and ECVPAR cell line.
8. YY water extract dereased the change of Ca^2+ in intracellular on ECV304 cell line and ECVPAR cell line.
According to the results, Yipahnsan showed to be a key antagonist of integrin α_vβ_3 and to be induction of apoptosts by p53 through flow cytometry. This report also demonstrated that expressions of MMP-9 and uPA were blocked under the angiogenesis model. Thus, we suggested that Yipahnsan blocks angiogenesis by inducing apoptosis in ECV304 and ECVPAR cell lines, and another oriental herbal medicine that treats qi-stagnation and hlood-stasis type also has angiogenic inhibition effects.
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